Part:BBa_K3020008:Design

Dnak+gfp

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 859
  

Design Notes

You need to use the proper annealing temperature in the PCR.

Source

The promoter Dnak is from Escherichia coli str. K-12 substr. MG1655.